9F2C
Archaeal histone protein HTkC from Thermococcus kodakarensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.655, 64.294, 79.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.840 |
| R-factor | 0.20508 |
| Rwork | 0.203 |
| R-free | 0.23649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.263 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmeas | 0.107 | 1.480 |
| Number of reflections | 24121 | 3815 |
| <I/σ(I)> | 12.63 | 1.36 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 7.16 | 7.15 |
| CC(1/2) | 0.998 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.01 M trisodium citrate and 33% PEG 6000 |
