9F22
DARPin eGFP complex DP1 (3G190.24)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.039, 77.039, 152.473 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.460 - 2.200 |
| R-factor | 0.17663 |
| Rwork | 0.174 |
| R-free | 0.22292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.402 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.196 | |
| Rmeas | 0.207 | |
| Rpim | 0.066 | 0.910 |
| Number of reflections | 20210127 | 2676 |
| <I/σ(I)> | 7.3 | 1.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 9.7 | |
| CC(1/2) | 0.997 | 0.415 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M Na acetate pH 5.5, 10% e/v PEG 8K, 10% PEG 1K, 0.2 M KBr |
