9F1V
Crystal structure of Borrelia burgdorferi CspZ-YA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-02 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.469, 41.552, 162.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.670 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.24106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.505 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 162.560 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.096 | 0.461 |
| Number of reflections | 17550 | 1116 |
| <I/σ(I)> | 15.5 | 4.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 13.2 | 13.5 |
| CC(1/2) | 0.998 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.2M Ammonium acetate 0.1 M sodium citrate pH 6.5 30% PEG 4000 |
