9F14
The crystal structure of full length tetramer CysB from Klebsiella aerogenes in complex with N-acetylserine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-11-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.87 |
Spacegroup name | H 3 2 |
Unit cell lengths | 185.864, 185.864, 113.043 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.817 - 2.300 |
Rwork | 0.211 |
R-free | 0.28980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.643 |
Data reduction software | HKL-3000 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.817 | 19.810 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.066 | 0.031 | 0.522 |
Rmeas | 0.072 | 0.034 | 0.572 |
Rpim | 0.029 | 0.015 | 0.233 |
Number of reflections | 33205 | 557 | 1251 |
<I/σ(I)> | 20 | ||
Completeness [%] | 99.8 | ||
Redundancy | 11.6 | 9.9 | 11.6 |
CC(1/2) | 0.999 | 0.999 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 12 -17% PEG 8000, 0.2M sodium tartrate and 0.1M Tris-HCl pH 8.5, 0.2M KSCN, 10 mM N-acetylserine |