9F0E
Bacterial histone protein HBb from Bdellovibrio bacteriovorus bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.697, 34.755, 56.433 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 42.490 - 1.850 |
| R-factor | 0.24959 |
| Rwork | 0.249 |
| R-free | 0.26744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.297 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.490 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmeas | 0.110 | 0.637 |
| Number of reflections | 16780 | 2747 |
| <I/σ(I)> | 9.59 | 2.18 |
| Completeness [%] | 93.0 | 95.2 |
| Redundancy | 4.68 | 4.7 |
| CC(1/2) | 0.996 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium sulfate, 0.1 M sodium acetate and 22% PEG 4000 |
