9F09
Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii. Complex with 2-deoxyribose, 7-Bromo-1H-imidazo[4,5-b]pyridine and 2'-deoxycytidine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2024-01-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976277 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 148.885, 148.885, 148.885 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.856 - 2.370 |
| Rwork | 0.184 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.695 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.856 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.090 | 1.480 |
| Rpim | 0.020 | 0.320 |
| Number of reflections | 22441 | 2337 |
| <I/σ(I)> | 33.3 | 3.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 41.7 | 43.5 |
| CC(1/2) | 1.000 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M sodium citrate; 20% PEG 3350 pH 7.0 |
