9F08

Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii. Covalent complex with 2-deoxyribose.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	120
Detector technology	PIXEL
Collection date	2024-01-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976277
Spacegroup name	I 21 3
Unit cell lengths	148.461, 148.461, 148.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.610 - 2.371
Rwork	0.199
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.661
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.610	2.460
High resolution limit [Å]	2.370	2.370
Rmerge	0.060	0.960
Rpim	0.010	0.220
Number of reflections	22224	2312
<I/σ(I)>	42.5	5.1
Completeness [%]	100.0
Redundancy	41.2
CC(1/2)	1.000	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	35% tascimate