9EZZ

Bacterial histone protein HBb from Bdellovibrio bacteriovorus bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.8856
Spacegroup name	P 21 21 21
Unit cell lengths	32.316, 34.223, 151.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.630 - 1.950
R-factor	0.22295
Rwork	0.221
R-free	0.26805
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.271
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.630	2.070
High resolution limit [Å]	1.950	1.950
Rmerge	0.097	2.020
Rmeas	0.104	2.160
Number of reflections	12919	2021
<I/σ(I)>	11.3	0.86
Completeness [%]	99.9	99.8
Redundancy	7.89	8.33
CC(1/2)	0.996	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES, pH 7.0, 0.2 M LiCl and 20% PEG 6k