9EZK

Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii (apo).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	120
Detector technology	PIXEL
Collection date	2024-01-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976277
Spacegroup name	I 21 3
Unit cell lengths	149.270, 149.270, 149.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.831 - 2.790
Rwork	0.172
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.838
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.940	2.940
High resolution limit [Å]	2.790	2.790
Rmerge	0.150	2.200
Rpim	0.030	0.490
Number of reflections	13954	2028
<I/σ(I)>	22.6	2.3
Completeness [%]	100.0
Redundancy	40.8
CC(1/2)	1.000	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	20 mM NaH2PO4 buffer; 0.2M sodium citrate; 20% (w/v) PEG 3350