9EZI
Structure of single-domain antibody VHH_h5 in complex with human Vsig4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 63 |
| Detector technology | PIXEL |
| Collection date | 2023-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.709, 50.877, 175.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.040 - 1.970 |
| R-factor | 0.1997 |
| Rwork | 0.198 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.952 | 2.000 |
| High resolution limit [Å] | 1.966 | 1.966 |
| Rmerge | 0.113 | 0.992 |
| Number of reflections | 17284 | 825 |
| <I/σ(I)> | 13.5 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 12.3 |
| CC(1/2) | 0.998 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0,1M Bis-Tris pH 6.5 and 30% PEG3350 |
