9EZB

SARS-CoV-2 Nucleocapsid N-terminal domain (NTD) mutant P67S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97598
Spacegroup name	P 21 21 21
Unit cell lengths	58.789, 92.466, 96.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.120 - 1.600
R-factor	0.2204
Rwork	0.220
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.390	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	69831	3345
<I/σ(I)>	11.2	2.2
Completeness [%]	99.7	97.1
Redundancy	10.3	9.7
CC(1/2)	0.996	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	150mM Sodium Chloride 28% PEG Smear Medium