9EYU

Human PRMT5 in complex with AZ compound 1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.95374
Spacegroup name	I 2 2 2
Unit cell lengths	101.063, 139.005, 178.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.350
R-factor	0.27667
Rwork	0.275
R-free	0.30497
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.653	2.590
High resolution limit [Å]	2.285	2.285
Number of reflections	27075	1354
<I/σ(I)>	7.1
Completeness [%]	92.3
Redundancy	6.8
CC(1/2)	0.995	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	14-16 w/v % PEG3350, 24-25 v/v % 2-methyl-2,4-pentanediol and 0.1 M tri-sodium-citrate buffer at pH 5.4