9EYE
A-SPECTRIN SH3 DOMAIN V9L, A11V, L31V, V44I, V53I MUTANT
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.007, 42.857, 49.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.451 - 1.130 |
| Rwork | 0.151 |
| R-free | 0.16880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.193 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.451 | 1.149 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.050 | 1.410 |
| Number of reflections | 26796 | 1193 |
| <I/σ(I)> | 13.7 | 0.8 |
| Completeness [%] | 99.0 | |
| Redundancy | 6 | |
| CC(1/2) | 0.999 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0M Ammonium Sulfate and 0.1M TRIS pH: 6.5 |
