9EYB
A-SPECTRIN SH3 DOMAIN V9L, A11V, V23L, M25C, V46W, V53L and V58L MUTANT
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-09-21 |
| Detector | STFC Large Pixel Detector |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.694, 36.949, 65.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.664 - 1.337 |
| Rwork | 0.141 |
| R-free | 0.17480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.940 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.664 | 1.360 |
| High resolution limit [Å] | 1.337 | 1.337 |
| Rmerge | 0.072 | |
| Number of reflections | 18004 | 901 |
| <I/σ(I)> | 16.4 | 1.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.7 | |
| CC(1/2) | 0.998 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.8M Ammonium Sulfate and 0.1M HEPES pH: 7.0 |
