9EY9
Yeast 20S proteasome in complex with a sybactin derivative (PheSyr)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.580, 301.800, 144.370 |
| Unit cell angles | 90.00, 112.65, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| R-factor | 0.19514 |
| Rwork | 0.193 |
| R-free | 0.24117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.097 | 0.691 |
| Number of reflections | 194950 | 17800 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 98.4 | 99.4 |
| Redundancy | 30 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |
