9EXY

Crystal structure of the PWWP1 domain of NSD2 bound by compound 34.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 31 2 1
Unit cell lengths	66.000, 66.000, 149.672
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.160 - 1.699
R-factor	0.2228
Rwork	0.221
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.160	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	41671	1719
<I/σ(I)>	11.5
Completeness [%]	92.2
Redundancy	8.4
CC(1/2)	0.999	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus screen, condition G9