9EXG

Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to Epsin-2 peptide (LIDL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	196
Detector technology	PIXEL
Collection date	2022-10-30
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	46.496, 94.289, 269.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.347 - 1.744
Rwork	0.191
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.532
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.347	67.260	1.770
High resolution limit [Å]	1.740	9.550	1.740
Rmerge	0.090	0.042	1.102
Rmeas	0.097	0.046	1.194
Rpim	0.037	0.018	0.457
Number of reflections	121808	884	5957
<I/σ(I)>	16.7
Completeness [%]	99.9
Redundancy	13.1	9.7	12.9
CC(1/2)	0.999	0.999	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	0.2M Lithium sulfate 0.1M Bis-Tris pH 5.5 25% (w/v) PEG3350