9EXF

Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to YAP1801 peptide (LIDM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	196
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	51.083, 89.739, 188.122
Unit cell angles	90.00, 90.39, 90.00

Refinement procedure

Resolution	65.013 - 1.950
Rwork	0.181
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.517
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.82))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.108	1.242
Rpim		0.553
Number of reflections	116855	5172
<I/σ(I)>	11	1.5
Completeness [%]	95.3	86.3
Redundancy	7.7	7.1
CC(1/2)	0.998	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.1M TRIS-HCl pH 8.5 0.28182M Lithium Sfate monohydrate 30% (w/v) PEG4000