9EX5

Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to Epsin-5 peptide (LIDL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	196
Detector technology	PIXEL
Collection date	2020-05-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	76.995, 133.449, 285.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.484 - 2.011
Rwork	0.182
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.428
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	142.760	142.760	2.050
High resolution limit [Å]	2.010	11.010	2.010
Rmerge	0.120	0.030	0.692
Rmeas	0.130	0.032	0.871
Rpim	0.049	0.012	0.517
Number of reflections	93922	681	1670
<I/σ(I)>	15.1
Completeness [%]	95.9
Redundancy	12.9	11.3	3.5
CC(1/2)	0.999	1.000	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	292	10 %(w/v) PEG 1000 10 %(w/v) PEG 8000