9EWP
Crystal structure of yeast E2 Ubiquitin-conjugating enzyme Ubc6 UBC domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-19 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.139, 44.169, 97.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.240 - 1.210 |
| R-factor | 0.1356 |
| Rwork | 0.135 |
| R-free | 0.15570 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.612 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.240 | 1.253 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Number of reflections | 109888 | 5615 |
| <I/σ(I)> | 25.47 | |
| Completeness [%] | 99.5 | |
| Redundancy | 12.3 | |
| CC(1/2) | 0.999 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG smear, ethylene glycol, ammonium sulphate, cadmium chloride hydrate |
