9EWE

DNA Polymerase Lambda I493K, E529D, Ca2+ Ground State Ternary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	55.801, 62.251, 138.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.550 - 3.040
R-factor	0.2441
Rwork	0.242
R-free	0.27910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.413
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.250	2.987
High resolution limit [Å]	2.737	2.737
Rpim	0.204	0.845
Number of reflections	8572	429
<I/σ(I)>	4.6	1.4
Completeness [%]	86.4
Redundancy	9.3
CC(1/2)	0.975	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	20 mM bicine pH7.5 300 mM Na-K tartrate 14% PEG 10K