9EWA
The sTeLIC pentameric Ligand-Gated Ion Channel (wild-type) in complex with 4-Bromophenethylamine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.919 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 143.210, 112.345, 210.796 |
| Unit cell angles | 90.00, 106.18, 90.00 |
Refinement procedure
| Resolution | 25.001 - 3.006 |
| Rwork | 0.241 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.001 | 3.117 |
| High resolution limit [Å] | 3.006 | 3.010 |
| Number of reflections | 63534 | 6069 |
| <I/σ(I)> | 6.94 | |
| Completeness [%] | 99.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.997 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 150 mM MgCl2 20mM Tris pH 8.0 35% PEG 200 |
