9EVR
Crystal structure of the kinetoplastid kinetochore protein KKT23 N-terminal domain from Trypanosoma brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2021-11-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 57.338, 57.338, 83.517 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.730 - 2.700 |
R-factor | 0.2463 |
Rwork | 0.245 |
R-free | 0.26490 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.009 |
RMSD bond angle | 1.064 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Arcimboldo |
Refinement software | PHENIX ((1.20_4444: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.730 | 42.730 | 2.530 |
High resolution limit [Å] | 2.490 | 6.750 | 2.490 |
Rmeas | 0.106 | 0.029 | 9.378 |
Number of reflections | 3165 | 199 | 148 |
<I/σ(I)> | 12.7 | 55.7 | 0.4 |
Completeness [%] | 100.0 | 100 | |
Redundancy | 18.4 | ||
CC(1/2) | 1.000 | 1.000 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1M Sodium phosphate, pH 6.5, 12% w/v PEG 8000 |