9EVR

Crystal structure of the kinetoplastid kinetochore protein KKT23 N-terminal domain from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	291
Detector technology	PIXEL
Collection date	2021-11-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 62 2 2
Unit cell lengths	57.338, 57.338, 83.517
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.730 - 2.700
R-factor	0.2463
Rwork	0.245
R-free	0.26490
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX ((1.20_4444: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.730	42.730	2.530
High resolution limit [Å]	2.490	6.750	2.490
Rmeas	0.106	0.029	9.378
Number of reflections	3165	199	148
<I/σ(I)>	12.7	55.7	0.4
Completeness [%]	100.0	100
Redundancy	18.4
CC(1/2)	1.000	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1M Sodium phosphate, pH 6.5, 12% w/v PEG 8000