9EVE

Crystal structure of the ARM domain of human ZNFX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976260
Spacegroup name	P 1 21 1
Unit cell lengths	46.850, 51.570, 86.650
Unit cell angles	90.00, 101.75, 90.00

Refinement procedure

Resolution	44.300 - 3.670
R-factor	0.2019
Rwork	0.198
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.570	4.020
High resolution limit [Å]	3.670	3.670
Rmerge	0.062	0.102
Rmeas	0.070
Rpim	0.026
Number of reflections	4452	961
<I/σ(I)>	17.6	12
Completeness [%]	97.3	89.8
Redundancy	6.6	6.5
CC(1/2)	0.998	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 8000, NaCl, citrate pH 4.2, glycerol