9EU8
The FK1 domain of FKBP51 in complex with SAFit-analog 15h
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 48.293, 48.293, 186.026 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.858 - 2.300 |
Rwork | 0.233 |
R-free | 0.25950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.902 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.510 | 46.510 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.144 | 0.111 | 1.284 |
Rmeas | 0.160 | 0.123 | 1.424 |
Rpim | 0.069 | 0.052 | 0.611 |
Number of reflections | 11940 | 269 | 1130 |
<I/σ(I)> | 7.5 | ||
Completeness [%] | 100.0 | ||
Redundancy | 9.6 | 8 | 10.1 |
CC(1/2) | 0.995 | 0.994 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG3350, 0.2M ammonium acetate, 0.1M HEPES-NaOH pH 7.5 |