9EU4

GH29A alpha-L-fucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8731
Spacegroup name	P 32 2 1
Unit cell lengths	140.372, 140.372, 110.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.190 - 2.330
R-factor	0.1929
Rwork	0.191
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.969
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.190	2.413
High resolution limit [Å]	2.330	2.330
Rmerge	0.104	1.000
Rmeas	0.106	1.000
Rpim	0.024	0.877
Number of reflections	54083	5354
<I/σ(I)>	14.42	0.3
Completeness [%]	98.2	82.36
Redundancy	20.7	21.3
CC(1/2)	0.998	0.337

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.15M Ammonium sulphate 0.1M Bis-tris pH 8.0 20% PEG 3350