9ETW
BTB domain of KLHL26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-31 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.740, 78.186, 95.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.090 - 1.510 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.770 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rpim | 0.055 | 0.857 |
| Number of reflections | 34811 | 1678 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.8 | |
| CC(1/2) | 0.998 | 0.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% PEG4000, 0.2M sodium acetate & 0.1M Tris pH 8.5 |
