9ETJ

Mouse CNPase catalytic domain with nanobody 10E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	101.730, 118.250, 122.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.950 - 2.550
R-factor	0.2542
Rwork	0.252
R-free	0.30440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.612
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.620
High resolution limit [Å]	2.550	2.550
Rmeas	0.112	3.496
Number of reflections	48792	3546
<I/σ(I)>	11.6	0.5
Completeness [%]	99.8	0.998
Redundancy	7.5	7.4
CC(1/2)	0.999	0.335

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Na malonate, 20% PEG3350