9ERT

Mouse CNPase catalytic domain with nano body 5E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 31 2 1
Unit cell lengths	110.832, 110.832, 83.281
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.990 - 2.750
R-factor	0.2929
Rwork	0.289
R-free	0.32320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.558
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	2.750	2.750
Rmeas	0.142	1.363
Number of reflections	10986	918
<I/σ(I)>	10.3	1.6
Completeness [%]	70.0	18
Redundancy	5.6	6.3
CC(1/2)	0.996	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M Na/K phosphate pH 7.0, 25% PEG3350