9ERG
Uhgb_MS mannoside synthase from an unknown human gut bacterium in complex with Mannose-1-Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979261 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.534, 83.368, 73.206 |
| Unit cell angles | 90.00, 106.35, 90.00 |
Refinement procedure
| Resolution | 45.184 - 2.100 |
| Rwork | 0.175 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.707 |
| Number of reflections | 40738 | 3664 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 96.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 285 |
