9EQK
WWP1 WW2-2,3-linker-WW3-WW4-HECT (WWP1-2L34H) with ordered WW2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-05 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 226.984, 59.719, 108.258 |
| Unit cell angles | 90.00, 99.41, 90.00 |
Refinement procedure
| Resolution | 84.490 - 3.000 |
| R-factor | 0.2089 |
| Rwork | 0.205 |
| R-free | 0.27440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.189 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.823 | 8.605 |
| High resolution limit [Å] | 3.000 | 3.171 |
| Rmerge | 0.200 | |
| Rmeas | 0.420 | |
| Rpim | 0.159 | |
| Number of reflections | 29046 | 1181 |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 94.5 | 100 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.975 | 0.368 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 100 mM Tris.HCl pH 6.5, 17.5 % reagent alcohol (90 % ethanol, 5 % methanol, 5 % isopropanol). |
