9EQB

Iron loaded mitochondrial ferritin H57A/E61A/E64A variant exposed to oxygen for 20 minutes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.975
Spacegroup name	F 4 3 2
Unit cell lengths	182.395, 182.395, 182.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.990 - 1.360
R-factor	0.1758
Rwork	0.174
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.822
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.990	1.409
High resolution limit [Å]	1.360	1.360
Number of reflections	56043	5464
<I/σ(I)>	0.58
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.998	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289	0.1 M bicine 2 M magnesium chloride 100 mM sodium chloride 60 mM ferrous chloride 3 mM sodium azide pH 9.0