9EQ8

Iron loaded mitochondrial ferritin exposed to oxygen for 2 minutes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.975
Spacegroup name	F 4 3 2
Unit cell lengths	182.770, 182.770, 182.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.870 - 1.480
R-factor	0.1661
Rwork	0.165
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.107
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.870	1.533
High resolution limit [Å]	1.480	1.480
Number of reflections	43961	4285
<I/σ(I)>	1.17
Completeness [%]	99.9
Redundancy	16.83
CC(1/2)	0.999	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	289	0.1 M bicine 2.0 M magnesium chloride 100 mM sodium chloride 60 mM ferrous chloride 3 mM sodium azide pH 9.0