9EPT

Crystal structure of the Plastid Redox Insensitive 2 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979260
Spacegroup name	P 41
Unit cell lengths	37.270, 37.270, 101.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.270 - 1.743
R-factor	0.1715
Rwork	0.169
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	XDS
Phasing software	Arcimboldo
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.270	1.790
High resolution limit [Å]	1.740	1.740
Rmerge	0.078	0.731
Number of reflections	14084	1041
<I/σ(I)>	20.55	3.23
Completeness [%]	99.8	99
Redundancy	10.91	8.91
CC(1/2)	0.999	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	6% PEG 8K, 0.2 M magnesium acetate, 0.1 M sodium cacodylate pH 6.5, 25% glycerol, protein concentration of 15 mg/mL.