9EOU
Crystal Structure of the b1b2 domains from Human Neuropilin-1 in complex with a peptide.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-16 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.690, 34.910, 69.970 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 42.570 - 1.550 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20249 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.879 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.570 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 45579 | 2252 |
| <I/σ(I)> | 1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium malonate pH 6.0, 9% w/v PEG 3350 |
