9EOI
Crystal structure of the GH19 endolysin from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.406, 79.696, 91.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.810 - 1.770 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.820 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.036 | 0.610 |
| Rmeas | 0.051 | 0.870 |
| Number of reflections | 47293 | 4592 |
| <I/σ(I)> | 10.1 | 1.1 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.990 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tri-Sodium Citrate dihydrate, 15% PEG3350 |
