9ENN

L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum in complex with N-epsilon-acetyl-L-lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	84.880, 132.120, 107.320
Unit cell angles	90.00, 95.93, 90.00

Refinement procedure

Resolution	47.380 - 1.800
R-factor	0.1553
Rwork	0.154
R-free	0.17690
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.858
Data reduction software	XDS (VERSION Jun 30, 2023 BUILT=20230630)
Data scaling software	XSCALE (VERSION Jun 30, 2023 BUILT=20230630)
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.800	1.800
Rmeas	0.080	1.844
Number of reflections	214609	14146
<I/σ(I)>	13.42	0.96
Completeness [%]	98.8	88
Redundancy	6.24	4.03
CC(1/2)	0.999	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein: 10 mg/ml; reservoir solution: 2.15 M (NH4)2SO4, 0.1 M sodium citrate, pH 7.0; drop with 200 nl protein + 200 nl reservoir