9ENK
L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum in complex with L-phenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-22 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.700, 131.730, 107.460 |
Unit cell angles | 90.00, 96.04, 90.00 |
Refinement procedure
Resolution | 40.670 - 2.200 |
R-factor | 0.1718 |
Rwork | 0.170 |
R-free | 0.20160 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.595 |
Data reduction software | XDS (VERSION Jun 30, 2023 BUILT=20230630) |
Data scaling software | XSCALE (VERSION Jun 30, 2023 BUILT=20230630) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.069 | 1.216 |
Number of reflections | 117122 | 8699 |
<I/σ(I)> | 19.43 | 2.27 |
Completeness [%] | 98.6 | 99.5 |
Redundancy | 7.15 | 7.34 |
CC(1/2) | 0.999 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | protein: 10 mg/ml; reservoir solution: 2.2 M (NH4)2SO4, 0.1 M sodium citrate, pH 6.4; drop with 500 nl protein + 500 nl reservoir |