9ENA

Lysosomal glucocerebrosidase in complex with a stabilizing nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	I 4 2 2
Unit cell lengths	113.209, 113.209, 257.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.860 - 1.700
R-factor	0.1645
Rwork	0.163
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.860	1.780
High resolution limit [Å]	1.700	1.700
Number of reflections	78278	3915
<I/σ(I)>	13.4
Completeness [%]	84.9
Redundancy	17.1
CC(1/2)	0.998	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.6 M Magnesium sulfate heptahydrate, 0.1 M MES pH6.5