9EN7

Hrp48 RRM1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	38.344, 45.496, 57.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.820 - 1.190
R-factor	0.163
Rwork	0.163
R-free	0.17030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.820	1.233
High resolution limit [Å]	1.190	1.190
Rmerge	0.065	1.536
Rmeas	0.068	1.597
Rpim	0.019	0.435
Number of reflections	32496	856
<I/σ(I)>	18.3	1.27
Completeness [%]	91.2	26.81
Redundancy	12.8	13.1
CC(1/2)	1.000	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	302	0.2 M K2SO4 and 20% (w/v) PEG 3350