9EN5

Crystal structure of yeast E2 Ubiquitin-conjugating enzyme Ubc6 UBC domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	44.020, 55.384, 76.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.800 - 1.330
R-factor	0.1599
Rwork	0.159
R-free	0.18560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.239
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	1.378
High resolution limit [Å]	1.330	1.330
Rmerge	0.039	1.390
Rmeas	0.040
Rpim		0.556
Number of reflections	40931	2752
<I/σ(I)>	31.1
Completeness [%]	94.2
Redundancy	12.1
CC(1/2)	1.000	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, di-ammonium hydrogen citrate