9EMG
SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with guanosine-5'-monophosphate as a product of c-di-GMP cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.340, 72.637, 82.940 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 43.393 - 1.148 |
| R-factor | 0.1129 |
| Rwork | 0.111 |
| R-free | 0.13110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.393 | 1.182 |
| High resolution limit [Å] | 1.148 | 1.148 |
| Rmerge | 0.045 | 0.567 |
| Rmeas | 0.054 | 0.701 |
| Number of reflections | 165597 | 8280 |
| <I/σ(I)> | 18.5 | 2.8 |
| Completeness [%] | 96.8 | |
| Redundancy | 6.6 | |
| CC(1/2) | 1.000 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 M Ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v Polyethylene glycol 5000. Protein concentration 7.5 mg/ml. Cryo-protection by glycerol 25 % v/v. |
