9EM5
OPR3 variant Y364P in its monomeric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.338, 89.941, 51.284 |
| Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.600 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.394 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.390 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rpim | 0.060 | 1.260 |
| Number of reflections | 54069 | 14664 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3 | |
| CC(1/2) | 0.990 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Tris-HCl (7.5), 50 mM sodium tartrate, 8-16% PEG8000 |
