9EM2
OPR3 wild type in its dimeric form obtained without sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.846, 89.734, 81.198 |
| Unit cell angles | 90.00, 109.17, 90.00 |
Refinement procedure
| Resolution | 46.670 - 1.540 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.046 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 1.560 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rpim | 0.040 | 1.720 |
| Number of reflections | 136455 | 4107 |
| <I/σ(I)> | 9.17 | |
| Completeness [%] | 99.5 | |
| Redundancy | 7 | |
| CC(1/2) | 0.990 | 0.130 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris/HCl (7.5), 50 mM sodium tartrate, 8-18% PEG8000 |
