9EL5

FANCM translocase domain bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97628
Spacegroup name	P 21 21 21
Unit cell lengths	80.940, 89.840, 93.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.240 - 2.200
R-factor	0.22224
Rwork	0.220
R-free	0.26697
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.323
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.240	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.083	1.236
Rmeas	0.086	1.283
Rpim	0.023	0.344
Total number of observations		41340
Number of reflections	35277	2994
<I/σ(I)>	16.6	2.2
Completeness [%]	100.0
Redundancy	13.5	13.8
CC(1/2)	0.999	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Sodium chloride, 0.1 M HEPES pH 7.0, 32.5% v/v PEG 400