9EKP

Crystal structure of WDR55 in complex with XS381774

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2024-06-25
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	78.316, 58.412, 87.218
Unit cell angles	90.00, 96.29, 90.00

Refinement procedure

Resolution	34.830 - 1.950
R-factor	0.15825
Rwork	0.156
R-free	0.20584
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.289
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.830	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.082	0.685
Rmeas	0.091	0.764
Rpim	0.041	0.335
Total number of observations		18967
Number of reflections	55823	3822
<I/σ(I)>	14.1	2.4
Completeness [%]	97.5
Redundancy	4.9	5
CC(1/2)	0.998	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% w/v PEG2000, 20% v/v ethylene glycol, 0.02M of each amino acid, 0.1M bicine/Trizma base pH 8.5