9EKM
Thomasclavelia ramosa Immunoglobulin A Protease Middle (Protease) Domain with C-terminal Domain #1 (Metal Chelated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.1808 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.740, 91.551, 86.848 |
| Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
| Resolution | 55.300 - 1.500 |
| R-factor | 0.1408 |
| Rwork | 0.139 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.700 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.089 | 1.174 |
| Number of reflections | 174570 | 7224 |
| <I/σ(I)> | 6.3 | 0.2 |
| Completeness [%] | 98.9 | 82.51 |
| Redundancy | 6.37 | 4.67 |
| CC(1/2) | 0.998 | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M Bis-Tris, pH 6.5 + 25% (w/v) PEG 3350 + 10 mM praseodymium (III) acetate |
