9EJN
Crystal structure of magnesium-transporting ATPase MgtA in an E1-like magnesium-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 180.403, 66.595, 218.666 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 24.910 - 3.550 |
| R-factor | 0.2905 |
| Rwork | 0.288 |
| R-free | 0.33900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.474 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.250 |
| High resolution limit [Å] | 3.200 | 3.210 |
| Number of reflections | 85323 | 7632 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.0 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.850 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4% tert-butanol, 100 mM MgSo4, 14% 2000 MME, 10% Glycerol, 100 mM MOPS pH 6.8 |
