9EIR

Ethylene-forming enzyme apoprotein from Penicillium digitatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127
Spacegroup name	P 21 21 21
Unit cell lengths	101.599, 115.880, 142.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.790 - 3.500
R-factor	0.2438
Rwork	0.242
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.431
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.790	3.780
High resolution limit [Å]	3.500	3.500
Number of reflections	21668	4376
<I/σ(I)>	5.1
Completeness [%]	99.6
Redundancy	6.2
CC(1/2)	0.947	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	10 % PEG 8000, 100 mM imidazole-HCl (pH 8.0), and 200 mM calcium acetate