9EHI
Crystal structure of ENL YEATS in complex with histone H3 methacrylated at K18
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.463, 98.413, 118.449 |
| Unit cell angles | 87.59, 90.00, 90.02 |
Refinement procedure
| Resolution | 37.180 - 2.600 |
| R-factor | 0.22173 |
| Rwork | 0.219 |
| R-free | 0.26653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.450 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.170 | 0.576 |
| Rmeas | 0.205 | 0.698 |
| Rpim | 0.115 | 0.390 |
| Total number of observations | 221400 | 13756 |
| Number of reflections | 72529 | 4523 |
| <I/σ(I)> | 9.8 | 2.4 |
| Completeness [%] | 97.8 | |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.971 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M MES monohydrate, pH 6.0, 20% (v/v) Jeffamine M600, pH 7.0 |
